Technologies
A High Performance (HP) Glycoanalysis System
High Throughput
High Resolution
High Sensitivity
High Reproducibility
High Reliability
HP-methods for sample preparation
Method & software for HP-glycoanalysis:
for data-processing
for data-analysis
Glycodatabase:
an oligosaccharide / glycan database
expandable, tailorable & integrable
Principles and Performance
- Based on multiplexed capillarygelelectro-phoresis with laser induced fluorescence detection (xCGE-LIF)
up to 96 capillaries in parallel enable “real” high throughput - Electrokinetic separation of glycocompounds in dependency of their charge, mass & shape
- Linear dynamic detection across a concentration-range of about 4 orders of magnitude
- No sample carryover due to exchange of separation matrix (gel) in front of each analysis
- Enables high performance glycoanalysis
- Up to 3500x more sensitive and 450x faster compared to standard-HPLC systems
- An order of magnitude higher separation power, compared to standard-HPLC systems
|
- High reproducibility of relative peak heights:
| RSD < 5% |
- Excellent reproducibility of normalized migration times:
| RSD < 0.03% |
| (shortterm / weeks) |
| RSD < 0.5%
(longterm / years) |
- highly efficient, robust, reproducible and highly sensitive “real” high-throughput
- Enables qualitative and quantitative high-performance glycoanalysis on 3 levels:
- Pattern comparison ("fingerprint"-analysis)
- Identification of compounds in complex mixtures via database matching (“glycoprofiling”)
- Extended structural analysis using exoglycosidase sequencing in combination with xCGE-LIF based glycoprofiling
