Besides state of the art glycoanalysis based on HILIC-FLR (the industry standard method) and mass spectrometry, glyXera also provides superior glycoanalysis through its proprietary high-throughput system that offers optimum performance and capacities compared to the glycoanalytical tools available elsewhere on the market:
HIGH-PERFORMANCE GLYCOANALYSIS
Our patented glyXboxCE system is based on multiplexed capillary gel electrophoresis with laser-induced fluorescence detection (xCGE-LIF), enabling generation and comparison of “glycofingerprints” from large sample sets and structural analysis of glycans, within these samples.
Based on xCGE-LIF, benefits include:
Up to 96 capillaries in parallel enable ‘real’ high
throughput
450x faster than conventional systems
Sensitivity more than 3,500x that of conventional systems
Separation power 15x greater than that of conventional
systems
High-reproducibility of peak heights
Excellent reproducibility of migration times
High-performance glycoanalysis is provided on three levels, comprising:
„
Glycofingerprinting
(GFP): glycosylation pattern analysis and comparison
„
Standard
glycoprofiling (STD-GP): identification of glycans in complex mixtures via
database matching
„
Extended
glycoprofiling (EXT-GP): extended structural analysis of glycans in complex
mixtures using exoglycosidase sequencing in combination with repeated xCGE-LIF-based glycoprofiling (including linkage
analysis)